HTR1A Polyclonal antibody

HTR1A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 55302-1-AP

Synonyms

5 HT 1A, 5 HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G 21, HTR1A, Serotonin receptor 1A

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Validation Data Gallery

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  IHC staining of human tonsillitis using 55302-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55302-1-AP (HTR1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55302-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55302-1-AP (HTR1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55302-1-AP targets HTR1A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: 5-hydroxytryptamine (serotonin) receptor 1A
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: NM_000524
• Gene Symbol: HTR1A
• Gene ID (NCBI): 3350
• RRID: AB_2881302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Flavonoids from mulberry leaves exhibit sleep-improving effects via regulating GABA and 5-HT receptors; Authors - Rui Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jorge (Verified Customer) (08-15-2022)

Tried a sample and was very happy with the results.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100