5HT2A Receptor Polyclonal antibody

5HT2A Receptor Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26438-1-AP

Synonyms

5 HT 2, 5 HT 2A, 5 HT2A, 5HT2A Receptor, HTR2, HTR2A, Serotonin receptor 2A

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Validation Data Gallery

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  WB analysis using 26438-1-AP

  H9C2 cells were subjected to SDS PAGE followed by western blot with 26438-1-AP (5HT2A Receptor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 26438-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 26438-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 26438-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 26438-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26438-1-AP (5HT2A Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: H9C2 cells
• Positive IHC detected in: human brain tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26438-1-AP targets 5HT2A Receptor in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: 5HT2A Receptor fusion protein Ag24056
• Full Name: 5-hydroxytryptamine (serotonin) receptor 2A
• Calculated Molecular Weight: 471 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC074848
• Gene Symbol: HTR2A
• Gene ID (NCBI): 3356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 5HT2A Receptor gene (HTR2A) codes for a G-protein coupled receptor and belongs to the G-protein coupled receptor 1 family. HTR2A plays a key role in serotonin pathway regulation. HTR2A affects neural activity, perception, cognition, and mood and functions as the main target of atypical antipsychotic drugs (PMID: 17691947, 21550210). 5HT2A Receptor has 2 isoforms with the molecular mass of 44 and 53 kDa.