HTR3A Polyclonal antibody

• Featured Product
• KD/KO Validated

HTR3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10443-1-AP

Synonyms

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

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Validation Data Gallery

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  WB analysis of mouse lung using 10443-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10443-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, human brain tissue
• Positive IHC detected in: human small intestine tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10443-1-AP targets HTR3A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTR3A fusion protein Ag0289
• Full Name: 5-hydroxytryptamine (serotonin) receptor 3A
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002354
• Gene Symbol: HTR3A
• Gene ID (NCBI): 3359
• RRID: AB_2124563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

Publications

Species	Application	Title
human	WB	Pain; Sex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp.; Authors - Loyd Dayna R DR
rat	WB, IHC	Front Pharmacol; Involvement of 5-Serotonin and Substance p Pathways in Dichroa Alkali Salt-Induced Acute Pica in Rats.; Authors - Lina Ma
rat	IF	Sci Rep; Oral Phyto-thymol ameliorates the stress induced IBS symptoms.; Authors - Selvaraj Subramaniyam
mouse	WB	Front Endocrinol (Lausanne); Electroacupuncture Attenuates Post-Inflammatory IBS-Associated Visceral and Somatic Hypersensitivity and Correlates With the Regulatory Mechanism of Epac1-Piezo2 Axis.; Authors - Jing Guo
mouse	WB	Biochem Biophys Res Commun; FSH signaling is involved in affective disorders.; Authors - Wen-Kai Bi
human	WB	Oncol Lett; Downregulation of 5-hydroxytryptamine receptor 3A expression exerts an anticancer activity against cell growth in colorectal carcinoma cells in vitro.; Authors - Jian Tang ; KD Validated