Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spleen tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13830-1-AP targets HTR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4742 Product name: Recombinant human HTR7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 263-445 aa of BC047526 Sequence: YKAARKSAAKHKFPGFPRVEPDSVIALNGIVKLQKEVEECANLSRLLKHERKNISIFKREQKAATTLGIIVGAFTVCWLPFFLLSTARPFICGTSCSCIPLWVERTFLWLGYANSLINPFIYAFFNRDLRTTYRSLLQCQYRNINRKLSAAGMHEALKLAERPERPEFVLQNADYCRKKGHDS Predict reactive species |
| Full Name | 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) |
| Calculated Molecular Weight | 445 aa, 49 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC047526 |
| Gene Symbol | HTR7 |
| Gene ID (NCBI) | 3363 |
| RRID | AB_2122706 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34969 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serotonin (5-hydroxytryptamine; 5-HT) acts as a neurotransmitter and neuromodulator in the central nervous system(CNS) (PMID:23100172). The behavioral effects of 5-HT are mediated by a large family of specific 5-HT receptors. HTR7 (5-hydroxytryptamine receptor 7) is a G protein-coupled receptor (GPCR), and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (PMID:34164474). It is located in different brain regions, such as the hypothalamus, thalamus, cerebral cortex, and hippocampus (PMID:29637287). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (PMID:9154233).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HTR7 antibody 13830-1-AP | Download protocol |
| WB protocol for HTR7 antibody 13830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 5-HT7 Receptor antibody (Cat# 13830-1-AP) has 8 citations. These appear in journals including Cell, Cell Reports Medicine, Cancer Letters, Molecular Psychiatry, Brain Research Bulletin, Biomedicines, Biochemical and Biophysical Research Communications, and Onco Targets and Therapy. Research fields span neuroscience and psychiatry (schizophrenia, depression, multiple sclerosis), oncology (colorectal cancer, NSCLC, biliary tract cancer), cilia spatial proteomics, and serotonin signaling. Applications include IF, IHC, and WB across human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Cancer Lett Serotonylation in tumor-associated fibroblasts contributes to the tumor-promoting roles of serotonin in colorectal cancer | ||
Biomedicines T Lymphocyte Serotonin 5-HT7 Receptor Is Dysregulated in Natalizumab-Treated Multiple Sclerosis Patients | ||
Brain Res Bull Research on the effects and related mechanisms of geomagnetic storm on depression |
Reviews
What customers say
The single available review rates the antibody 4 out of 5 stars. The user tested it at a 1:1000 dilution on rabbit spinal cord tissue for both immunohistochemistry and immunofluorescence, reporting it as a good antibody that stained well when paired with a goat anti-Rabbit IgG (H+L) Texas Red-X secondary antibody. Overall, the feedback is positive, with the reviewer confirming reliable staining performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Clarissa (Verified Customer) (05-08-2019) | Good antibody, stained with goat anti-Rabbit IgG (H+L) Secondary Antibody, Texas Red-X.
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