HTRA1 Polyclonal antibody

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• HTRA1 Polyclonal antibody (55011-1-AP)

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• KD/KO Validated

HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55011-1-AP Copied!

Publications (23)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.21.-, HtrA, PRSS11

• 

  WB analysis using 55011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HAP1 cells using 55011-1-AP

  HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis of mouse brain using 55011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 55011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HAP1 cells using 55011-1-AP

  HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 55011-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HTRA1 antibody (55011-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HAP1 cells, rat brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55011-1-AP targets HTRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: HtrA serine peptidase 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: NM_002775
• Gene Symbol: HTRA1
• Gene ID (NCBI): 5654
• RRID: AB_10859830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92743
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

Publications

What published studies show

The HTRA1 antibody (Cat# 55011-1-AP) has 23 citations across journals including Cell, J Clin Invest, Biomaterials, Cancer Biol Med, Am J Pathol, FASEB J, iScience, Commun Biol, Sci Rep, Cell Mol Life Sci, and others. Research fields span aging proteomics, Parkinson's disease, gastric cancer, retinal degeneration, blood-brain barrier disruption, cardiac remodeling, bone biology, coronary artery disease, osteoarthritis, CARASIL syndrome, head and neck cancer, and cerebral ischemia/reperfusion injury.

Automatically generated from the 23 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cell; Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures; Authors - Yingjie Ding
human, mouse	WB	J Clin Invest; N-acetyl-l-leucine lowers α-synuclein levels and improves synaptic function in Parkinson's disease models.; Authors - Pingping Song
mouse		Biomaterials; Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm; Authors - Craig Jun Kit Wong
human	WB	Cancer Biol Med; VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy.; Authors - Yanna Bi
mouse,human	WB,IF	Cell Commun Signal; Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration; Authors - Wenchang Xu
mouse	IF	Int J Mol Med; Endothelial NOX4‑driven oxidative stress inhibition reverses HtrA1 deficiency‑induced blood‑brain barrier disruption and cognitive impairment.; Authors - Shi-Na Song