HTRA2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83492-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human CD100 were performed. The affinity constant is 0.574 nM.

HTRA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83492-7-PBS

Clone No.240496D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240496D1, EC:3.4.21.108, HtrA serine peptidase 2, OMI, Omi stress-regulated endoprotease

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Product Information

83492-7-PBS targets HTRA2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8455

  Product name: Recombinant human HTRA2 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 133-458 aa of BC000096

  Sequence: AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

• Full Name: HtrA serine peptidase 2
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: BC000096
• Gene Symbol: HTRA2
• Gene ID (NCBI): 27429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43464
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.