Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19957-1-AP

Synonyms

HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15



Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19957-1-AP targets Huntingtin in IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name huntingtin
Calculated molecular weight 348 kDa
GenBank accession numberNM_002111
Gene symbol HTT
Gene ID (NCBI) 3064
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HTT, also named as HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. The antibody recognizes the C-term of HTT.

Protocols

Product Specific Protocols
IHC protocol for Huntingtin antibody 19957-1-APDownload protocol
IF protocol for Huntingtin antibody 19957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols