HUS1B Polyclonal antibody

HUS1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18229-1-AP

Synonyms

Checkpoint protein HUS1B, hHUS1B, HUS1B, RP11 532F6.1

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Validation Data Gallery

• 

  WB analysis of RAW264.7 using 18229-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18229-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 18229-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, RAW264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18229-1-AP is HUS1B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HUS1B fusion protein Ag12712
• Full Name: HUS1 checkpoint homolog b (S. pombe)
• Calculated Molecular Weight: 278 aa, 31 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC101697
• Gene Symbol: HUS1B
• Gene ID (NCBI): 135458
• RRID: AB_10694671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.