Validation Data Gallery
|Positive WB detected in||Daudi cells, HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue, human lung tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
19430-1-AP targets HUWE1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HUWE1 fusion protein Ag13763|
|Full Name||HECT, UBA and WWE domain containing 1|
|Calculated molecular weight||437 aa, 482 kDa|
|Observed molecular weight||482 kDa|
|GenBank accession number||BC002602|
|Gene ID (NCBI)||10075|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).
WNK1 is an assembly factor for the human ER membrane protein complex.
Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.
HUWE1 promotes EGFR ubiquitination and degradation to protect against renal tubulointerstitial fibrosis.
J Cell Sci
Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).
J Cell Sci
The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.
Biochem Biophys Res Commun
NEDD4L-mediated LTF protein degradation limits ferroptosis.