HVCN1 Polyclonal antibody

HVCN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14162-1-AP

Synonyms

VSOP, Voltage-gated hydrogen channel 1, Voltage sensor domain-only protein, UNQ578/PRO1140, Hydrogen voltage-gated channel 1

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Validation Data Gallery

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  WB analysis of Raji using 14162-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14162-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14162-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 14162-1-AP

  IP result of anti-HVCN1 (IP:14162-1-AP, 4ug; Detection:14162-1-AP 1:300) with PC-3 cells lysate 4000 ug.

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  IHC staining of human tonsillitis using 14162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 14162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 14162-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 14162-1-AP (HVCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

14162-1-AP targets HVCN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HVCN1 fusion protein Ag5350
• Full Name: hydrogen voltage-gated channel 1
• Calculated Molecular Weight: 273 aa, 32 kDa
• Observed Molecular Weight: 28-32 kDa, ~60 kDa
• GenBank Accession Number: BC032672
• Gene Symbol: HVCN1
• Gene ID (NCBI): 84329
• RRID: AB_2878023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW ~60 kDa.

Publications

Species	Application	Title
human	IHC	Front Immunol; Diagnostic and Predictive Value of Immune-Related Genes in Crohn's Disease.; Authors - Bing Yu