HVEM/TNFRSF14 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of human urine using 81078-6-RR (same clone as 81078-6-PBS)

  human urine sample was subjected to SDS PAGE followed by western blot with 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81078-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 81078-6-RR (same clone as 81078-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81078-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 81078-6-RR (same clone as 81078-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81078-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81078-6-RR

  Biolayer interferometry (BLl) kinetic assays of 81078-6-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 3.00 nM.

HVEM/TNFRSF14 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 81078-6-PBS

Clone No.250728B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

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Product Information

81078-6-PBS targets HVEM/TNFRSF14 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1275

  Product name: recombinant human TNFRSF14 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 39-202 aa of BC002794

  Sequence: LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV

• Full Name: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002794
• Gene Symbol: TNFRSF14
• Gene ID (NCBI): 8764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92956
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.