HYAL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HYAL2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15115-1-AP

Synonyms

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

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Validation Data Gallery

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  WB analysis using 15115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15115-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15115-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15115-1-AP

  Immunofluorescent analysis of Hela cells, using HYAL2 antibody 15115-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15115-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HYAL2 antibody (15115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, Raji cells, mouse liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15115-1-AP targets HYAL2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HYAL2 fusion protein Ag7157
• Full Name: hyaluronoglucosaminidase 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC000692
• Gene Symbol: HYAL2
• Gene ID (NCBI): 8692
• RRID: AB_2123393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publications

Species	Application	Title
mouse	WB,IF	Genet Med; Elucidating the clinical spectrum and molecular basis of HYAL2 deficiency.; Authors - James Fasham ; KO Validated
human	IHC,IF,WB	Front Immunol; Complement protein C1q stimulates hyaluronic acid degradation via gC1qR/HABP1/p32 in malignant pleural mesothelioma; Authors - Andrea Balduit
MOUSE	WB	PLoS Genet; PMID: 28081210; Authors - Martina M A Muggenthaler ; KO Validated
mouse	WB	Dermatol Ther; Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice.; Authors - Keiichi Hiramoto

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