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HYAL4 Polyclonal antibody

HYAL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18139-1-AP

Synonyms

Hyal 4, HYAL4, hyaluronoglucosaminidase 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HYAL4 Polyclonal antibody (18139-1-AP)

    WB analysis using 18139-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18139-1-AP (HYAL4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using HYAL4 Polyclonal antibody (18139-1-AP)

    WB analysis of NIH/3T3 using 18139-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18139-1-AP (HYAL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using HYAL4 Polyclonal antibody (18139-1-AP)

    IP experiment of mouse testis using 18139-1-AP

    IP result of anti-HYAL4 (IP:18139-1-AP, 4ug; Detection:18139-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL4 Polyclonal antibody (18139-1-AP)

    IHC staining of human testis using 18139-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL4 Polyclonal antibody (18139-1-AP)

    IHC staining of human testis using 18139-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HYAL4 Polyclonal antibody (18139-1-AP)

    IHC staining of human placenta using 18139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HYAL4 Polyclonal antibody (18139-1-AP)

    IHC staining of human placenta using 18139-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18139-1-AP (HYAL4 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inK-562 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18139-1-AP targets HYAL4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYAL4 fusion protein Ag12766
Full Name hyaluronoglucosaminidase 4
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC104788
Gene Symbol HYAL4
Gene ID (NCBI) 23553
RRIDAB_2878506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronic acid (HA), a glycosaminoglycan that is ubiquitously present in the extracellular space of higher animals, maintains matrix structure and controls cellular functions such as proliferation, differentiation, and locomotion. Hyal enzymes that catabolize HA, are involved in development and tumorigenesis.HYAL4, a member of the Hyal family, is a chondroitin sulfate (CS)-specific endo-β-acetylgalactosaminidase. The expression of hHYAL4 mRNA is not ubiquitous but restricted to placenta, skeletal muscle, and testis (PMID: 23086929). This polyclonal antibody raised against 29-460aa of human HYAL4. The apparent molecular weight is larger than the calculated molecular weight of 54 kDa, which is likely due to posttranslational modification, presumably by glycosylation (PMID: 19889881).

Protocols

Product Specific Protocols
WB protocol for HYAL4 antibody 18139-1-APDownload protocol
IHC protocol for HYAL4 antibody 18139-1-APDownload protocol
IP protocol for HYAL4 antibody 18139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols