HYDIN Polyclonal antibody

HYDIN Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24741-1-AP

Synonyms

HYDIN, HYDIN1, HYDIN2, KIAA1864

---

Validation Data Gallery

• 

  IHC staining of human testis using 24741-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24741-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cerebellum using 24741-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cerebellum using 24741-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive IHC detected in: human testis tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24741-1-AP is HYDIN Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HYDIN fusion protein Ag10732
• Full Name: hydrocephalus inducing homolog (mouse)
• Calculated Molecular Weight: 721aa,82 kDa; 5120aa,560 kDa
• GenBank Accession Number: BC028351
• Gene Symbol: HYDIN
• Gene ID (NCBI): 54768
• RRID: AB_2879700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HYDIN (hydrocephalus inducing homolog), also named as HYDIN1 or HYDIN2, is a 5121 amino acid protein, which localizes in the cell projection HYDIN exists as seven alternatively spliced isoforms and is expressed in the ciliated ependymal cell layer of heart ventricle. HYDIN is also found in ciliated epithelial cells of developing spermatocytes, oviduct and bronchi and is required for ciliary motility. HYDIN gene mutation is involved in Ciliary dyskinesia primary 5.