Hexokinase 2 Recombinant antibody

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  WB analysis using 85875-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85875-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Hexokinase 2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85875-1-RR

Clone No.250190C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HCT 116 cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85875-1-RR targets Hexokinase 2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Full Name: hexokinase 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102-120 kDa
• GenBank Accession Number: BC021116
• Gene Symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52789
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).