ChromoTek Histone-Chromobody® plasmid (eGFP)

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

EGFP

Epitope

Histone

Cat no : tcg

Synonyms

Chromobody, Histone Chromobody



Product Information

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

ApplicationsLive cell imaging
SpecificityHistone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterEGFP
Codon usage Mammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-H2A-H2B single domain antibody (sdAb) fragment
SequenceWith the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Transfectionransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Commun

Helicase LSH/Hells regulates kinetochore function, histone H3/Thr3 phosphorylation and centromere transcription during oocyte meiosis.

Authors - Claudia Baumann