Phospho-Histone H2B (Ser14) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83811-1-RR (same clone as 83811-1-PBS)

  Non-treated NIH/3T3 cells and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83811-1-RR (Phospho-Histone H2B (Ser14)) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83811-1-PBS in a different storage buffer formulation.

Phospho-Histone H2B (Ser14) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83811-1-PBS

Clone No.240570A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

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Product Information

83811-1-PBS targets Phospho-Histone H2B (Ser14) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 2, H2be
• Calculated Molecular Weight: 14 kDa
• GenBank Accession Number: BC005827
• Gene Symbol: Histone H2B
• Gene ID (NCBI): 8349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16778
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. In addition, MST1 is known to phosphorylate histone H2B in vitro and has recently been shown to directly phosphorylate histone H2B at Ser14 in vivo. (PMID: 17548476)