HuR/ELAVL1 Recombinant antibody

HuR/ELAVL1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4L10

Cat no : 82690-2-RR

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

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Validation Data Gallery

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  WB analysis using 82690-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82690-2-RR (HuR/ELAVL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HCT 116 cells, NCI-H1299 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82690-2-RR targets HuR/ELAVL1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HuR/ELAVL1 fusion protein Ag2249
• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 30-37 kDa
• GenBank Accession Number: BC003376
• Gene Symbol: ELAVL1
• Gene ID (NCBI): 1994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.