Human VISTA/C10orf54 ELISA Kit
Cat no : KE00282
Synonyms
C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412
Validation Data Gallery
Product Information
KE00282 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VISTA/C10orf54 ELISA kit is to be used to detect and quantify protein levels of endogenous VISTA/C10orf54. The assay recognizes human VISTA/C10orf54. An antibody specific for VISTA/C10orf54 has been pre-coated onto the microwells. The VISTA/C10orf54 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for VISTA/C10orf54 is added to detect the captured VISTA/C10orf54 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VISTA/C10orf54 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 64115 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 100% | 86%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.76 | 0.23 | 4.9 |
| 2 | 20 | 1.12 | 0.05 | 4.7 |
| 3 | 20 | 0.32 | 0.02 | 6.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.20 | 0.22 | 4.2 |
| 2 | 24 | 1.19 | 0.05 | 4.1 |
| 3 | 24 | 0.31 | 0.03 | 10.8 |
Background Information
VISTA (V-domain Ig suppressor of T cell activation), also known as C10orf54, B7-H5, PD-1H and Dies1, is a member of the immune checkpoints of the B7 family that shares significant homology with PD-L1 and PD-L2. VISTA is predominantly expressed on haematopoietic cells, myeloid cells, and weakly expressed on T cells. It can act as a ligand expressed on antigen presenting cells (APC), and also functions as an coinhibitory receptor for CD4+ T cells. VISTA exerts negative regulatory functions by suppressing the activation, proliferation, and cytokine secretion of T cells. VISTA plays an important role in tumor progression and immune escape and can be used as a predictor for diagnosis or immunotherapy of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.