Human ADAMTS13 ELISA Kit
Cat no : KE00286
Synonyms
ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP
Validation Data Gallery
Product Information
KE00286 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ADAMTS13 ELISA kit is to be used to detect and quantify protein levels of endogenous human ADAMTS13. The assay recognizes human ADAMTS13. An antibody specific for human ADAMTS13 has been pre-coated onto the microwells. The human ADAMTS13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ADAMTS13 is added to detect the captured human ADAMTS13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ADAMTS13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.0028 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11093 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 84%-120% |
| Cell culture supernatant | 89% | 71%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 20.76 | 1.05 | 5.04 |
| 2 | 20 | 4.96 | 0.25 | 5.07 |
| 3 | 20 | 1.14 | 0.06 | 5.01 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 23.54 | 1.98 | 8.39 |
| 2 | 24 | 5.50 | 0.29 | 5.23 |
| 3 | 24 | 1.29 | 0.08 | 6.52 |
Background Information
A disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) is a multidomain metalloprotease for which until now only a single substrate has been identified. ADAMTS13 cleaves the polymeric force-sensor von Willebrand factor (VWF) that unfolds under shear stress and recruits platelets to sites of vascular injury. In patients with immune-mediated thrombotic thrombocytopenic purpura (iTTP), a rare life threatening disease, ADAMTS13 is targeted by autoantibodies that inhibit its activity or promote its clearance. In the absence of ADAMTS13, VWF polymers are not adequately processed, resulting in spontaneous adhesion of blood platelets, which presents as severe, life-threatening microvascular thrombosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADAM TS 13, ADAM TS13, ADAMTS 13, ADAMTS13, C9orf8, DKFZp434C2322, FLJ42993, TTP, vWF cleaving protease, vWF CP, VWFCP |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Blood Vessel Thromb Hemost Elevated VWF with normal ADAMTS13 in pediatric sickle cell disease: links to crises and ischemic stroke. |