Human AFP ELISA Kit
Cat no : KE00310
Synonyms
Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein
Validation Data Gallery
Product Information
KE00310 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AFP ELISA kit is to be used to detect and quantify protein levels of endogenous human AFP. The assay recognizes human AFP. An antibody specific for human AFP has been pre-coated onto the microwells. The human AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AFP is added to detect the captured human AFP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AFP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 6.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 174 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 108% | 92%-124% |
| Cell culture supernatant | 92% | 81%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 835.8 | 22.8 | 2.7 |
| 2 | 20 | 204.9 | 4.4 | 2.2 |
| 3 | 20 | 45.3 | 1.9 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 804.2 | 25.4 | 3.2 |
| 2 | 24 | 201.5 | 5.3 | 2.6 |
| 3 | 24 | 50.2 | 3.4 | 6.8 |
Background Information
AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma levels decrease rapidly after birth. AFP is one of the earliest markers of the hepatocyte lineage. High AFP concentrations have been correlated with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.