Speedy™ Human AFP One-Step ELISA Kit
Cat no : SE50003
Synonyms
PCSK9, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
Validation Data Gallery
Product Information
| Product name | Speedy™ Human AFP One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 7.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 174 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 85%-120% |
| Cell culture supernatant | 110% | 102%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 513.9 | 6.9 | 1.3 |
| 2 | 8 | 137.5 | 4.5 | 3.3 |
| 3 | 8 | 70.7 | 2.6 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 528.9 | 17.5 | 3.3 |
| 2 | 16 | 140.3 | 4.5 | 3.2 |
| 3 | 16 | 71.8 | 2.4 | 3.3 |
Background Information
AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma levels decrease rapidly after birth. AFP is one of the earliest markers of the hepatocyte lineage. High AFP concentrations have been correlated with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PCSK9, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin |