Human AGT ELISA Kit
Cat no : KE00332
Synonyms
Angiotensinogen, Angiotensinogen/AGT, Serpin A8, SERPINA8, Alpha 1 antiproteinase
Validation Data Gallery
Product Information
KE00332 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AGT ELISA kit is to be used to detect and quantify protein levels of endogenous human AGT. The assay recognizes human AGT. An antibody specific for human AGT has been pre-coated onto the microwells. The human AGT protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AGT is added to detect the captured human AGT protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AGT ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 183 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 90%-119% |
| Human plasma | 96% | 83%-110% |
| Urine | 95% | 77%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.67 | 0.17 | 3.56 |
| 2 | 20 | 1.24 | 0.04 | 3.62 |
| 3 | 20 | 0.31 | 0.03 | 9.05 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.74 | 0.12 | 2.60 |
| 2 | 24 | 1.24 | 0.05 | 4.43 |
| 3 | 24 | 0.32 | 0.03 | 9.71 |
Background Information
Angiotensinogen(AGT), a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiotensinogen, Angiotensinogen/AGT, Serpin A8, SERPINA8, Alpha 1 antiproteinase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
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