Human AGXT ELISA Kit
Cat no : KE00513
Synonyms
AGXT,AGT,AGT1,AGXT1,Alanine--glyoxylate aminotrans
Validation Data Gallery
Product Information
KE00513 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AGXT ELISA kit is to be used to detect and quantify protein levels of endogenous human AGXT. The assay recognizes human AGXT. An antibody specific for human AGXT has been pre-coated onto the microwells. The human AGXT protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AGXT is added to detect the captured human AGXT protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AGXT ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.188-12 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 189 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 102% | 94%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.91 | 0.19 | 3.28 |
| 2 | 8 | 1.31 | 0.02 | 1.75 |
| 3 | 8 | 0.70 | 0.02 | 2.30 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.98 | 0.22 | 3.62 |
| 2 | 16 | 1.33 | 0.04 | 2.80 |
| 3 | 16 | 0.71 | 0.02 | 2.51 |
Background Information
AGXT (Alanine-glyoxylate aminotransferase) is mostly localized in the peroxisomes and is involved in glyoxylate detoxification. Knocking down of AGXT in the hepatocellular carcinoma (HCC) cell line could induce a cell cycle shift from G0/G1 to S and G2/M together with enhanced cell proliferation, increased cell death, and migration. Moreover, a systematic analysis of liver tissues from patients with primary hyperoxaluria revealed high rates of chronic liver disease, hepatic inflammation and fibrosis in patients with AGXT dysfunction
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGXT,AGT,AGT1,AGXT1,Alanine--glyoxylate aminotrans |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.