Human AK2 ELISA Kit
Cat no : KE00959
Synonyms
adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2, ATP:AMP phosphotransferase
Validation Data Gallery
Product Information
KE00959 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AK2 ELISA kit is to be used to detect and quantify protein levels of endogenous human AK2. The assay recognizes human AK2. An antibody specific for human AK2 has been pre-coated onto the microwells. The human AK2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AK2 is added to detect the captured human AK2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AK2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 204 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 79%-87% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.20 | 0.37 | 3.61 |
| 2 | 8 | 2.52 | 0.10 | 3.78 |
| 3 | 8 | 0.67 | 0.02 | 3.54 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.05 | 0.36 | 3.54 |
| 2 | 16 | 2.47 | 0.11 | 4.34 |
| 3 | 16 | 0.66 | 0.03 | 3.93 |
Background Information
AK2(Adenylate kinase 2, mitochondrial ) is also named ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation. AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP. Defects in AK2 are the cause of reticular dysgenesis (RDYS). It has 6 isoforms produced by alternative splicing.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2, ATP:AMP phosphotransferase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.