Human AKAP1 ELISA Kit
Cat no : KE00908
Synonyms
AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, DAKAP1
Validation Data Gallery
Product Information
KE00908 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AKAP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human AKAP1. The assay recognizes human AKAP1. An antibody specific for human AKAP1 has been pre-coated onto the microwells. The human AKAP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AKAP1 is added to detect the captured human AKAP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AKAP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 4.7-300 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8165 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 93%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 188.3 | 7.1 | 3.8 |
| 2 | 8 | 41.0 | 2.4 | 5.9 |
| 3 | 8 | 23.1 | 1.3 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 193.4 | 9.9 | 5.1 |
| 2 | 16 | 41.7 | 2.5 | 6.1 |
| 3 | 16 | 22.1 | 1.7 | 7.6 |
Background Information
AKAP1 (A-Kinase Anchoring Protein 1), also known as D-AKAP1 or AKAP149, is a mitochondrial and endoplasmic reticulum (ER)-anchored scaffolding protein that plays a critical role in organizing signaling complexes, particularly those involving protein kinase A (PKA). By tethering PKA and other signaling molecules to specific subcellular compartments, AKAP1 regulates essential cellular processes such as mitochondrial dynamics, apoptosis, metabolism, and oxidative stress response. AKAP1 contains a dual-specificity domain that binds both type I and type II regulatory subunits of PKA, along with additional binding sites for phosphatases, phosphodiesterases, and other kinases. Its localization at the outer mitochondrial membrane (OMM) allows it to modulate mitochondrial function, including fission/fusion balance and reactive oxygen species (ROS) management. Studies have linked AKAP1 to cardiovascular diseases, neurodegenerative disorders, and cancer, highlighting its importance in cellular homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, DAKAP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.