Human ARK5 ELISA Kit
Cat no : KE00767
Synonyms
NUAK1, AMPK-related protein kinase 5, EC:2.7.11.1, NUAK 1, NUAK family SNF1-like kinase 1
Validation Data Gallery
Product Information
KE00767 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ARK5 ELISA kit is to be used to detect and quantify protein levels of endogenous human ARK5. The assay recognizes human ARK5. An antibody specific for human ARK5 has been pre-coated onto the microwells. The human ARK5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ARK5 is added to detect the captured human ARK5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ARK5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9891 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 80%-86% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.81 | 0.78 | 9.96 |
| 2 | 8 | 1.28 | 0.08 | 6.06 |
| 3 | 8 | 0.60 | 0.03 | 4.82 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.50 | 0.57 | 10.30 |
| 2 | 16 | 1.24 | 0.09 | 7.02 |
| 3 | 16 | 0.64 | 0.06 | 10.03 |
Background Information
AMPK-related protein kinase 5 (ARK5) is a serine/threonine kinase that has been identified as the fifth member of the AMP-activated protein kinase (AMPK) family. ARK5-mediated Akt was established as a key element that functions as a survival factor in the complex tumorigenesis network. ARK5 serves a role in the metastasis and invasion of colorectal (CRC) cancer, pancreatic cancer (PC), gastric cancer, hepatic cancer and squamous cell carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NUAK1, AMPK-related protein kinase 5, EC:2.7.11.1, NUAK 1, NUAK family SNF1-like kinase 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.