Human ARNT/HIF-1 beta ELISA Kit
Cat no : KE00895
Synonyms
ARNT, HIF-1 beta, HIF1B, bHLHe2, Class E basic helix-loop-helix protein 2
Validation Data Gallery
Product Information
KE00895 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ARNT/HIF-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous human ARNT/HIF-1 beta. The assay recognizes human ARNT/HIF-1 beta. An antibody specific for human ARNT/HIF-1 beta has been pre-coated onto the microwells. The human ARNT/HIF-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ARNT/HIF-1 beta is added to detect the captured human ARNT/HIF-1 beta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ARNT/HIF-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 405 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 117% | 116%-118% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.26 | 0.19 | 3.64 |
| 2 | 8 | 1.30 | 0.05 | 3.65 |
| 3 | 8 | 0.74 | 0.03 | 3.99 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.13 | 0.24 | 4.74 |
| 2 | 16 | 1.29 | 0.05 | 3.79 |
| 3 | 16 | 0.74 | 0.03 | 3.83 |
Background Information
HIF1B (HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. HIF1B plays a pivotal role in the adaptive responses to (micro-)environmental stresses such as oxygen deprivation (hypoxia).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ARNT, HIF-1 beta, HIF1B, bHLHe2, Class E basic helix-loop-helix protein 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.