Human ASGR1 ELISA Kit
Cat no : KE00548
Synonyms
ASGR1, asialoglycoprotein receptor 1, HL-1, ASGP R 1, ASGPR
Validation Data Gallery
Product Information
KE00548 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ASGR1 ELISA kit is to be used to detect and quantify protein levels of endogenous human ASGR1. The assay recognizes human ASGR1. An antibody specific for human ASGR1 has been pre-coated onto the microwells. The human ASGR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ASGR1 is added to detect the captured human ASGR1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ASGR1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 432 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 84%-111% |
| Cell lysate | 85% | 77%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 557.9 | 36.9 | 6.6 |
| 2 | 8 | 120.4 | 4.8 | 4.0 |
| 3 | 8 | 37.2 | 2.1 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 546.7 | 39.7 | 7.3 |
| 2 | 16 | 121.2 | 5.7 | 4.7 |
| 3 | 16 | 37.3 | 2.2 | 6.0 |
Background Information
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses, including hepatitis B, and is also a target for liver-specific drug delivery.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ASGR1, asialoglycoprotein receptor 1, HL-1, ASGP R 1, ASGPR |