Human ATG12 ELISA Kit
Cat no : KE00892
Synonyms
APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12
Validation Data Gallery
Product Information
KE00892 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ATG12 ELISA kit is to be used to detect and quantify protein levels of endogenous human ATG12. The assay recognizes human ATG12. An antibody specific for human ATG12 has been pre-coated onto the microwells. The human ATG12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ATG12 is added to detect the captured human ATG12 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATG12 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9140 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 117% | 112%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 768.5 | 21.7 | 2.8 |
| 2 | 8 | 177.2 | 5.7 | 3.2 |
| 3 | 8 | 96.9 | 4.3 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 743.0 | 40.5 | 5.5 |
| 2 | 16 | 174.6 | 8.5 | 4.9 |
| 3 | 16 | 94.7 | 4.6 | 4.8 |
Background Information
ATG12, also named as APG12 and APG12L, it is required for autophagy. Autophagy is an intracellular protein degradation process in which cytoplasmic components, including organelles, are encapsulated in double-membrane autophagosomes and transported to lysosomes or vacuoles for degradation. During autophagy, ATG12 binds to ATG5 through a ubiquitin-like covalent linkage system, and the formed ATG12-ATG5 complex further promotes the binding of ATG8 to phosphatidylethanolamine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.