Human ATG9A ELISA Kit
Cat no : KE00582
Synonyms
APG9L1, Autophagy-related protein 9A, mATG9
Validation Data Gallery
Product Information
KE00582 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ATG9A ELISA kit is to be used to detect and quantify protein levels of endogenous human ATG9A. The assay recognizes human ATG9A. An antibody specific for human ATG9A has been pre-coated onto the microwells. The human ATG9A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ATG9A is added to detect the captured human ATG9A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATG9A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.24 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 79065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 94% | 75%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 50.79 | 1.85 | 3.64 |
| 2 | 8 | 13.92 | 0.67 | 4.81 |
| 3 | 8 | 7.48 | 0.50 | 6.68 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 51.22 | 1.60 | 3.12 |
| 2 | 16 | 13.97 | 0.59 | 4.24 |
| 3 | 16 | 7.40 | 0.46 | 6.21 |
Background Information
ATG9A, a transmembrane protein of the core autophagy pathway, cycles between the Golgi, endosomes and a vesicular compartment. It is involved in autophagosome assembly and enables phospholipid scramblase activity. ATG9A is expressed ubiquitously across various tissues, with high expression in the brain and spinal cord. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APG9L1, Autophagy-related protein 9A, mATG9 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.