Human ATIC ELISA Kit
Cat no : KE01013
Synonyms
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR, AICAR formyltransferase, AICAR transformylase/inosine monophosphate cyclohydrolase, AICARFT
Validation Data Gallery
Product Information
KE01013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ATIC ELISA kit is to be used to detect and quantify protein levels of endogenous human ATIC. The assay recognizes human ATIC. An antibody specific for human ATIC has been pre-coated onto the microwells. The human ATIC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ATIC is added to detect the captured human ATIC protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ATIC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 471 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 77%-92% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 22.48 | 1.60 | 7.12 |
| 1 | 8 | 5.81 | 0.48 | 8.19 |
| 3 | 8 | 1.94 | 0.07 | 3.43 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 24.12 | 2.20 | 9.14 |
| 2 | 16 | 5.85 | 0.56 | 9.64 |
| 3 | 16 | 2.09 | 0.20 | 9.74 |
Background Information
Bifunctional purine biosynthesis protein ATIC(also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. ATIC has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR, AICAR formyltransferase, AICAR transformylase/inosine monophosphate cyclohydrolase, AICARFT |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.