Human Alpha-2-macroglobulin ELISA Kit
Cat no : KE00855
Synonyms
A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha 2M, Alpha-2-M
Validation Data Gallery
Product Information
KE00855 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Alpha-2-macroglobulin ELISA kit is to be used to detect and quantify protein levels of endogenous human Alpha-2-macroglobulin. The assay recognizes human Alpha-2-macroglobulin. An antibody specific for human Alpha-2-macroglobulin has been pre-coated onto the microwells. The human Alpha-2-macroglobulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Alpha-2-macroglobulin is added to detect the captured human Alpha-2-macroglobulin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Alpha-2-macroglobulin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Saliva, Human Milk, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 80%-97% |
| Urine | 101% | 94%-109% |
| Saliva | 104% | 101%-109% |
| Human Milk | 96% | 94%-99% |
| Cell lysate | 106% | 95%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.00 | 0.21 | 4.22 |
| 2 | 8 | 1.22 | 0.02 | 1.40 |
| 3 | 8 | 0.65 | 0.01 | 1.62 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.17 | 0.29 | 5.56 |
| 2 | 16 | 1.31 | 0.10 | 7.76 |
| 3 | 16 | 0.65 | 0.01 | 2.29 |
Background Information
Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha 2M, Alpha-2-M |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.