Human Angiopoietin 1 ELISA Kit

Sensitivity

17.3 pg/mL

Range

62.5 - 4000 pg/mL

Reactivity

Human

Cat no : KE00212

Synonyms

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003, PDGFAB



Product Information

KE00212 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 1. The assay recognizes human Angiopoietin 1. An antibody specific for Angiopoietin 1 has been pre-coated onto the microwells. The Angiopoietin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Angiopoietin 1 is added to detect the captured human Angiopoietin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Angiopoietin 1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Saliva
Assay type Sandwich
Sensitivity 17.3 pg/mL
Range 62.5 - 4000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum98%80%-116%
Human saliva89%71%-109%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,021.594.54.7
220557.813.42.4
320155.86.64.2

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,845.259.93.2
224544.920.93.8
324161.15.93.6

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer and other tumors.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003, PDGFAB