Human Angiopoietin 2 ELISA Kit

Sensitivity

4.6 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00226

Synonyms

AGPT2, ANG 2, ANG2, angiopoietin 2, Angiopoietin-2, ANGPT2, PDGFAB



Product Information

KE00226 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 2 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 2. The assay recognizes Human Angiopoietin 2. An antibody specific for Angiopoietin 2 has been pre-coated onto the microwells. The Angiopoietin 2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Angiopoietin 2 is added to detect the captured Angiopoietin 2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human Angiopoietin 2 ELISA Kit
Tests 1 X 96 well plate
Sample type Cell culture supernatants
Assay type Sandwich
Sensitivity 4.6 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell culture supernatants100%77%-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12032.71.03.2
220122.44.43.6
320495.220.44.1

InterAssay

Samplenmean ( pg/mL)SDCV%
12431.61.34.1
224120.93.63.0
324512.123.54.6

Background Information

Angiopoietin 2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin 2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin 2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin 2 is expressed only at sites of vascular remodeling. Angiopoietin 2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AGPT2, ANG 2, ANG2, angiopoietin 2, Angiopoietin-2, ANGPT2, PDGFAB