Human Aquaporin 4 ELISA Kit
Cat no : KE00486
Synonyms
AQP4, AQP 4, AQP-4, Aquaporin4, Aquaporin-4
Validation Data Gallery
Product Information
KE00486 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Aquaporin 4 ELISA kit is to be used to detect and quantify protein levels of endogenous human Aquaporin 4. The assay recognizes human Aquaporin 4. An antibody specific for human Aquaporin 4 has been pre-coated onto the microwells. The human Aquaporin 4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Aquaporin 4 is added to detect the captured human Aquaporin 4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Aquaporin 4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cerebrospinal fluid |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 361 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 76% | 72%-79% |
| Human cerebrospinal fluid (CSF) | 105% | 100%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,496.0 | 71.7 | 2.9 |
| 2 | 8 | 556.4 | 33.0 | 5.9 |
| 3 | 8 | 294.5 | 9.5 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,580.0 | 108.0 | 4.2 |
| 2 | 16 | 557.9 | 27.3 | 4.9 |
| 3 | 16 | 287.4 | 18.3 | 6.4 |
Background Information
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. AQP4 is the principal water channel protein in the CNS, predominantly expressed in astrocyte endfeet and ependymal cells. It regulates water balance in brain tissue. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AQP4, AQP 4, AQP-4, Aquaporin4, Aquaporin-4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.