Human B2M ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00303
Synonyms
B2M, beta 2 microglobulin, HDCMA22P
Validation Data Gallery
Product Information
KE00303 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human B2M ELISA kit is to be used to detect and quantify protein levels of endogenous human B2M. The assay recognizes human B2M. An antibody specific for human B2M has been pre-coated onto the microwells. The human B2M protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human B2M is added to detect the captured human B2M protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human B2M ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 567 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 94%-118% |
| Urine | 92% | 82%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.23 | 0.14 | 3.26 |
| 2 | 20 | 1.01 | 0.02 | 2.09 |
| 3 | 20 | 0.24 | 0.01 | 3.10 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.19 | 0.11 | 2.55 |
| 2 | 24 | 1.02 | 0.02 | 2.33 |
| 3 | 24 | 0.27 | 0.01 | 4.07 |
Background Information
Beta-2-microglobulin (B2M) is a 12-kDa protein comprised of 99 amino acids. B2M is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Serum B2M is regarded as a marker of disease severity in renal injury, infections, amyloidosis, and aging-related diseases. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases including multiple myeloma, lymphoma, and solid tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B2M, beta 2 microglobulin, HDCMA22P |