Speedy™ Human B7-H3/CD276 One-Step ELISA Kit
Cat no : SE50017
Synonyms
CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3
Validation Data Gallery
Product Information
.
| Product name | Speedy™ Human B7-H3/CD276 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 80381 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 89%-115% |
| Cell culture supernatant | 110% | 105%-120% |
| Urine | 102% | 93%-115% |
| Cell lysate | 101% | 86%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 709.5 | 14.4 | 2.0 |
| 2 | 8 | 161.6 | 5.2 | 3.2 |
| 3 | 8 | 85.7 | 1.8 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 728.7 | 28.2 | 3.9 |
| 2 | 16 | 166.7 | 7.9 | 4.7 |
| 3 | 16 | 81.3 | 5.6 | 6.8 |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor. Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy. Soluble form of B7-H3 (sB7-H3), released by monocytes, dendritic cells, activated T cells, and B7-H3-positive tumor cells, has been detected in human blood samples and cell culture supernatants.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.