Human BACH1 ELISA Kit
Cat no : KE00597
Synonyms
BACH 1, BACH-1
Validation Data Gallery
Product Information
KE00597 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human BACH1 ELISA kit is to be used to detect and quantify protein levels of endogenous human BACH1. The assay recognizes human BACH1. An antibody specific for human BACH1 has been pre-coated onto the microwells. The human BACH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human BACH1 is added to detect the captured human BACH1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human BACH1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 571 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 116% | 108%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,707.2 | 69.0 | 4.0 |
| 2 | 8 | 362.5 | 11.3 | 3.1 |
| 3 | 8 | 198.0 | 8.8 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,593.1 | 139.4 | 8.7 |
| 2 | 16 | 357.4 | 13.8 | 3.9 |
| 3 | 16 | 195.9 | 7.1 | 3.6 |
Background Information
BTB and CNC homology 1 (BACH1) is a redox-sensitive transcription factor that binds antioxidant response elements and is known for its ability to suppress heme oxygenase 1 transcription. BACH1 is known to regulate multiple physiological processes including heme homeostasis, oxidative stress response, senescence, cell cycle, and mitosis. BACH1 is highly expressed in tumors including breast and lung, relative to their non-tumor tissues. In a tumor, BACH1 promotes invasion and metastasis of cancer cells, and the expression of BACH1 presents a poor outcome for cancer patients including breast and lung cancer patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BACH 1, BACH-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.