Human BRAP ELISA Kit
Cat no : KE00495
Synonyms
BRAP,BRAP2,BRCA1 associated protein,BRCA1-associat
Validation Data Gallery
Product Information
KE00495 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human BRAP ELISA kit is to be used to detect and quantify protein levels of endogenous human BRAP. The assay recognizes human BRAP. An antibody specific for human BRAP has been pre-coated onto the microwells. The human BRAP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human BRAP is added to detect the captured human BRAP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human BRAP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8315 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 98%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.61 | 0.13 | 2.88 |
| 2 | 8 | 1.16 | 0.03 | 2.44 |
| 3 | 8 | 0.33 | 0.02 | 7.06 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.56 | 0.15 | 3.28 |
| 2 | 16 | 1.15 | 0.03 | 2.93 |
| 3 | 16 | 0.33 | 0.02 | 6.33 |
Background Information
BRCA1-Associated Protein (BRAP), also known as BRAP2 and RNF52. The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm (PMID: 32890957). BRCA1-associated protein (BRAP) can bind to breast cancer suppressor protein BRCA1. It functions as an E3 ubiquitin ligase and could modulate mitogen-activated protein kinase (MAPK) signaling pathway (PMID: 33240929).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BRAP,BRAP2,BRCA1 associated protein,BRCA1-associat |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.