Human BRCA1 ELISA Kit
Cat no : KE00487
Synonyms
BRCA1, BRCC1, BROVCA1, EC:2.3.2.27, IRIS
Validation Data Gallery
Product Information
KE00487 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human BRCA1 ELISA kit is to be used to detect and quantify protein levels of endogenous human BRCA1. The assay recognizes human BRCA1. An antibody specific for human BRCA1 has been pre-coated onto the microwells. The human BRCA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human BRCA1 is added to detect the captured human BRCA1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human BRCA1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 672 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 106% | 89%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,262.3 | 147.3 | 6.5 |
| 2 | 8 | 559.5 | 38.4 | 6.9 |
| 3 | 8 | 269.5 | 10.0 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,247.2 | 155.6 | 6.9 |
| 2 | 16 | 553.5 | 40.9 | 7.4 |
| 3 | 16 | 265.0 | 12.1 | 4.5 |
Background Information
The BRCA1 gene is located on the long arm of chromosome 17. It comprises 24 exons and encodes an 1863 amino acid protein, which contains multiple domains, each of them associated with one or more specific functions. BRCA1 contributes to a variety of cellular processes, including DNA repair, DNA replication stress reduction, cell cycle checkpoint, transcriptional regulation, chromatin remodeling, and ubiquitination.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BRCA1, BRCC1, BROVCA1, EC:2.3.2.27, IRIS |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.