Speedy™ Human BST2 One-Step ELISA Kit
Cat no : SE50090
Synonyms
CD317, Bone marrow stromal antigen 2, BST 2, BST-2, CD 317
Validation Data Gallery
Product Information
| Product name | Speedy™ Human BST2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 23.4 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 684 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 85% | 74%-102% |
| Cell lysate | 94% | 81%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,677.5 | 35.1 | 1.3 |
| 2 | 8 | 683.5 | 6.9 | 1.0 |
| 3 | 8 | 349.2 | 5.4 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,648.6 | 49.7 | 1.9 |
| 2 | 16 | 687.7 | 8.6 | 1.3 |
| 3 | 16 | 353.3 | 6.7 | 1.9 |
Background Information
BST2 (Bone Marrow Stromal Cell Antigen 2), also known as Tetherin, CD317, or HM1.24, is a type II transmembrane glycoprotein widely involved in immunomodulation and antiviral defense. It restricts viral release and transmission by binding nascent viral particles to the host cell membrane. In addition, BST2 is highly expressed in a variety of human cancers and is closely associated with tumor cell proliferation, invasion, migration, and immune escape. For example, in laryngeal squamous cell carcinoma, high expression of BST2 correlates with poorer patient prognosis. BST2 is also involved in cell membrane integrity and lipid raft formation, and can activate the epidermal growth factor receptor signaling pathway, thus playing a role in tumorigenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD317, Bone marrow stromal antigen 2, BST 2, BST-2, CD 317 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.