Human Beta galactosidase ELISA Kit
Cat no : KE00478
Synonyms
GLB1,Acid beta-galactosidase,Beta galactosidase,Be
Validation Data Gallery
Product Information
KE00478 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Beta galactosidase ELISA kit is to be used to detect and quantify protein levels of endogenous human Beta galactosidase. The assay recognizes human Beta galactosidase. An antibody specific for human Beta galactosidase has been pre-coated onto the microwells. The human Beta galactosidase protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Beta galactosidase is added to detect the captured human Beta galactosidase protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Beta galactosidase ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2720 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 89%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.41 | 0.64 | 4.77 |
| 2 | 8 | 3.02 | 0.15 | 4.98 |
| 3 | 8 | 1.44 | 0.07 | 4.73 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 13.56 | 0.75 | 5.56 |
| 2 | 16 | 2.88 | 0.23 | 8.06 |
| 3 | 16 | 1.48 | 0.08 | 5.45 |
Background Information
β-Galactosidase (GLB1), also named ELNR1 or Lactase, is a type of glycoside hydrolase that is widely found in plants, animals, and microorganisms. It can catalyze the hydrolysis of β-galactoside compounds to generate monosaccharides and plays a crucial role in lactose metabolism. Additionally, it is extensively used in the study of cellular aging. For example, increased activity of SA-β-gal (senescence-associated β-galactosidase) can be used as a marker for senescent cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GLB1,Acid beta-galactosidase,Beta galactosidase,Be |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.