Human C1QC ELISA Kit
Cat no : KE00100
Synonyms
Complement C1q C chain,C1Q C,C1qC,C1QG
Validation Data Gallery
Product Information
KE00100 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human C1QC ELISA kit is to be used to detect and quantify protein levels of endogenous human C1QC. The assay recognizes human C1QC. A monoclonal antibody specific for human C1QC has been pre-coated onto the microwells. The human C1QC protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for human C1QC is added to detect the captured human C1QC protein. For signal development,horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human C1QC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 125 - 8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 714 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 85% | 73%-111% |
| Cell culture supernatant | 90% | 74%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6,688.8 | 428.8 | 6.4 |
| 2 | 20 | 1,810.7 | 102.7 | 5.7 |
| 3 | 20 | 171.3 | 11.0 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6,298.3 | 445.6 | 7.1 |
| 2 | 20 | 1,728.5 | 137.1 | 7.9 |
| 3 | 20 | 164.1 | 9.2 | 5.6 |
Background Information
The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. C1q plays an important role in complement activation by immune complexes. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Complement C1q C chain,C1Q C,C1qC,C1QG |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.