Human C1QTNF2 ELISA Kit
Cat no : KE01015
Synonyms
Complement C1q tumor necrosis factor-related protein 2, CTRP2, UNQ6349/PRO21054
Validation Data Gallery
Product Information
KE01015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human C1QTNF2 ELISA kit is to be used to detect and quantify protein levels of endogenous human C1QTNF2. The assay recognizes human C1QTNF2. An antibody specific for human C1QTNF2 has been pre-coated onto the microwells. The human C1QTNF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human C1QTNF2 is added to detect the captured human C1QTNF2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human C1QTNF2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 114898 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 85%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.68 | 0.44 | 7.70 |
| 2 | 8 | 1.16 | 0.05 | 4.16 |
| 3 | 8 | 0.54 | 0.03 | 5.84 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.14 | 0.66 | 12.90 |
| 2 | 16 | 1.15 | 0.05 | 4.01 |
| 3 | 16 | 0.59 | 0.06 | 10.75 |
Background Information
C1QTNF2 (C1q and Tumor Necrosis Factor Related Protein 2) is a secretory protein that belongs to the C1q/TNF-related protein (CTRP) family. It is also commonly known as CTRP2. This family of proteins shares structural similarities with two important protein superfamilies: the immune complement component C1q and tumor necrosis factor (TNF)-α.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Complement C1q tumor necrosis factor-related protein 2, CTRP2, UNQ6349/PRO21054 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.