Human C5AR1/CD88 ELISA Kit
Cat no : KE00741
Synonyms
C5AR, C5AR1, C5A, C5a anaphylatoxin chemotactic receptor 1, C5a R
Validation Data Gallery
Product Information
KE00741 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human C5AR1/CD88 ELISA kit is to be used to detect and quantify protein levels of endogenous human C5AR1/CD88. The assay recognizes human C5AR1/CD88. An antibody specific for human C5AR1/CD88 has been pre-coated onto the microwells. The human C5AR1/CD88 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human C5AR1/CD88 is added to detect the captured human C5AR1/CD88 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human C5AR1/CD88 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 728 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 92%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.27 | 0.98 | 9.50 |
| 2 | 8 | 2.49 | 0.13 | 5.07 |
| 3 | 8 | 1.28 | 0.07 | 5.66 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.09 | 0.89 | 8.82 |
| 2 | 16 | 2.34 | 0.20 | 8.60 |
| 3 | 16 | 1.25 | 0.08 | 6.23 |
Background Information
C5a anaphylatoxin chemotactic receptor 1 (C5AR1, also known as CD88) is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. It is involved in several processes, including the complement component C5a signaling pathway and the positive regulation of the ERK1 and ERK2 cascade. C5AR1 is activated upon binding of the C5a molecule, stimulating chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. It plays a crucial role in host defense and immune responses, contributing to inflammation and tissue repair.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C5AR, C5AR1, C5A, C5a anaphylatoxin chemotactic receptor 1, C5a R |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.