Human C7 ELISA Kit
Cat no : KE00552
Synonyms
complement component 7, Complement component C7
Validation Data Gallery
Product Information
KE00552 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human C7 ELISA kit is to be used to detect and quantify protein levels of endogenous human C7. The assay recognizes human C7. An antibody specific for human C7 has been pre-coated onto the microwells. The human C7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human C7 is added to detect the captured human C7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human C7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 730 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 87%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,267.3 | 70.3 | 5.5 |
| 2 | 8 | 295.9 | 12.5 | 4.2 |
| 3 | 8 | 152.2 | 6.2 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,204.1 | 98.1 | 8.1 |
| 2 | 16 | 292.8 | 17.4 | 5.9 |
| 3 | 16 | 152.5 | 6.7 | 4.4 |
Background Information
The complement system is an important effector that bridges the innate and adaptive immune systems. Complement component 7 (C7) is a single-chain plasma glycoprotein that is the final product of the complement cascade and plays a central role in the activation of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 is a potential tumor suppressor and a prognostic biomarker for prostate cancer and renal cell carcinoma. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | complement component 7, Complement component C7 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.