Human CBX1 ELISA Kit
Cat no : KE00484
Synonyms
CBX1,CBX,Chromobox protein homolog 1,Heterochromat
Validation Data Gallery
Product Information
KE00484 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CBX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human CBX1. The assay recognizes human CBX1. An antibody specific for human CBX1 has been pre-coated onto the microwells. The human CBX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CBX1 is added to detect the captured human CBX1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CBX1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10951 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 113% | 103%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.17 | 0.54 | 5.93 |
| 2 | 8 | 2.52 | 0.03 | 1.09 |
| 3 | 8 | 1.20 | 0.08 | 6.71 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.52 | 0.59 | 6.15 |
| 2 | 16 | 2.41 | 0.13 | 5.57 |
| 3 | 16 | 1.27 | 0.09 | 7.36 |
Background Information
CBX1 (Chromobox protein homolog 1), also known as CBX. It is expected to be located in nucleus, the protein is expressed in all adult and embryonic tissues. It is a member of the heterochromatin protein family. CBX1 is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. It may play an important role in the epigenetic control of chromatin structure and gene expression. The calculated molecular weight of CBX1 is 21 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CBX1,CBX,Chromobox protein homolog 1,Heterochromat |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.