Human CCL11/Eotaxin ELISA Kit
Cat no : KE00256
Synonyms
CCL11, Eotaxin, C C motif chemokine 11, C-C motif chemokine 11, CCL 11
Validation Data Gallery
Product Information
KE00256 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL11 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL11.The assay recognizes human CCL11. An antibody specific for CCL11 has been pre-coated onto the microwells. The CCL11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL11 is added to detect the captured human CCL11 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL11/Eotaxin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 15.6 - 1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6356 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 75%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 406.6 | 40.7 | 10.0 |
| 2 | 20 | 99.7 | 6.5 | 6.6 |
| 3 | 20 | 24.7 | 1.8 | 7.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 456.1 | 27.1 | 5.9 |
| 2 | 24 | 107.3 | 5.3 | 4.9 |
| 3 | 24 | 23.9 | 2.0 | 8.4 |
Background Information
CCL11 (also known as eotaxin-1), a CC chemokine, is involved in the selective recruitment of eosinophils into inflammatory sites during allergic reactions, and this chemokine is extensively examined in asthma, allergic rhinitis and other eosinophil-related conditions. CCL-11 production is induced by T helper (Th)-2 cytokines, like IL-13 (Interleukin-13), IL-10 (Interleukin-10) and IL-4 (Interleukin-4). It is a product of eosinophils, B-cells, fibroblasts, endothelial cells, macrophages, chondrocytes and other cells. The main receptor for CCL11 is CCR3, but CCL11 also binds CCR2 and CCR5.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL11, Eotaxin, C C motif chemokine 11, C-C motif chemokine 11, CCL 11 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Drug Resist Updat Stabilin-1(+) lipid-associated macrophages promote lung adenocarcinoma liver metastasis and osimertinib resistance through impairing macrophage phagocytosis via SIRPα-CD47 axis. |