Human CCL17/TARC ELISA Kit
Cat no : KE00213
Synonyms
A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC
Validation Data Gallery
Product Information
KE00213 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL17/TARC ELISA kit is to be used to detect and quantify protein levels of endogenous CCL17/TARC. The assay recognizes human CCL17/TARC. An antibody specific for CCL17/TARC has been pre-coated onto the microwells. The CCL17/TARC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL17/TARC is added to detect the captured human CCL17/TARC protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL17/TARC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 7.8 - 500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6361 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 92% | 75%-105% |
| Cell Culture supernatants | 101% | 75%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 13.0 | 0.4 | 3.2 |
| 2 | 20 | 56.4 | 3.7 | 6.5 |
| 3 | 20 | 251.2 | 9.6 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 14.5 | 0.7 | 4.7 |
| 2 | 24 | 59.8 | 2.7 | 4.4 |
| 3 | 24 | 233.1 | 14.8 | 6.4 |
Background Information
CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Pyromeconic acid-enriched Erigeron annuus water extract as a cosmetic ingredient for itch relief and anti-inflammatory activity | ||
Am J Pathol Single-Cell and Spatial Transcriptomics-Based Research Reveals Association Between M2a Macrophages and Tumor Spread through Air Spaces in Lung Adenocarcinoma. |